It was detected for the ability of a local fungus T.harzianum and A.alternate which was isolated from the rhizosphere of Wheat plant Triticum aestivum L. to produce the enzyme complex cellulase in solid medium (CMC-Agar). So it has been showed that T.harzianum and A.alternate have an ability to produce this enzyme, While the T.harzianum have higher production of cellulase than A.alternate. Then conducted a quantitative test using Mandelium liquid medium is used to determine the optimal conditions (incubation time, temperature, PH) for the best cellulase production of the two fungus studied. It was, the best conditions for its production, from T.harzianum is 7 days inchubation (3.97 U/mL), temperature 26C (4.07 U/mL) and pH=5.5 (4.19U/mL). While the best conditions for its production from A.alternate is 8 days inchubation (3.29U/mL), temperature 28C (3.15 U/mL) and pH=5.0 (3.86 U/mL). The comparison between the enzymatic activity average for both fungus , was showed superiority T.harzianum by production cellulase than fungus A.alternate. And discern that the effect of the incubation time on cellulase production from fungus T.harzianum more than its effect on cellulase production from fungus A.alternate. While effect of pH and temperature on cellulase production from fungus T.harzianum less than their effect on cellulase production from fungus A.alternate.