Background: socket preservation is a main therapeutic procedure to prevent severe marginal bone resorption after tooth extraction. The aim of this study is to investigate if there is any difference in the amount of new bone formation 4 months after extraction and ridge preservation compared to that after 6 months, Using tricalcium phosphate as a bone substitute. Methods: Minimally traumatic extraction with socket preservation using tricalcium phosphate was done at 30 single-rooted tooth sites in 28 patients. 15 sites healed for an average of 18 weeks (early healing group), and 17 sites were allowed to heal for an average of 27 weeks (delayed healing group) before collecting the bone samples . histological analysis was held to detect the percentage of new bone formation, residual substitute particles, and connective tissue/non-mineralized structures for each site. Results: All samples revealed a new bone formation, most of the residual substitute particles were surrounded by woven bone. No statistically significant differences in the amount of newly formed bone or residual substitute particles were found between the two groups. Overall, the early healing group demonstrated a mean of34.8% newbone, 20.5%residual substitute material, and 44.7% non-mineralized tissue. The delayed healing group showed mean values of 36%, 18.5%, and 45.5%, respectively. Conclusion: This study suppose that waiting for 6 months after tooth extraction and socket preservation using tricalcium phosphate does not provide a greater amount of new bone formation or less residual bone particles compared to that after 4 months.