Chronic periodontitis (CP) is an inflammatory disease, characterized by gingivitis, and affecting tooth supporting tissues, forming periodontal pockets with associated attachment loss, alveolar bone resorption. It is common in adults, but can also occur at any age. The rate of disease progression may be influenced by local, systemic conditions, and/or environmental factors that alter the normal host response to bacterial plaque, and affect the susceptibility to disease.It is suggested that periodontitis is partially associated by genetic factors, that many genes are involved in inflammation susceptibility, mainly include the vitamin D receptor (VDR) gene that is implicated in bone metabolism and the host immune response. 80 Syrian subjects were recruited for vitamin D receptor gene polymorphism study, and allocated in two groups: 50 diagnosed with CP and mean age was (64 ± 0.722) years, 30 matched controls. DNA was isolated from peripheral blood cells, and genotyping was performed by polymerase chain reaction (PCR) method and restriction fragment length polymorphism analysis (RFLP) by using FokI enzyme. By using Chi square test, no significant differences were found between the study groups in the frequencies of alleles and genotypes at FokI position of VDR gene, age and sex. These findings suggest that the investigated factors are not associated with periodontal disease in this studied sample of Syrian population.